Abu Agila Mohammad Mas'ud Kheir Al-Marimi was taken into custody about two years after former US Attorney General Bill Barr first announced the United States filed charges against him.
A Justice Department spokesperson confirmed to Reuters on Sunday that the United States has custody of the suspect. Mas'ud is expected to make his initial court appearance in a federal court in Washington.
Details about the timing of the hearing will be forthcoming, the spokesperson added.
Court documents described Mas'ud as an expert bombmaker who joined Libya's External Security Organization intelligence service in the 1970s and took part in a number of operations outside Libya, reaching the rank of colonel.
A military source in the Libyan city of Misrata said Mas'ud had been flown from the airport there. Reuters could not immediately establish when.
The families of those killed in the Lockerbie bombing have been told the suspect is in US custody, a spokesperson for Scotland's Crown Office and Procurator Fiscal Service (COPFS) said on Sunday.
The BBC first reported Mas'ud's arrest.
The bomb on board the Boeing 747, which was flying from London to New York City, killed all 259 people on board and 11 on the ground, the deadliest ever militant attack in Britain.
Reuters