US-listed shares of Alibaba rose 3.5% in trading before the bell.
The six units will include Cloud Intelligence Group, Taobao Tmall Commerce Group, Local Services Group, Cainiao Smart Logistics Group, Global Digital Commerce Group and Digital Media and Entertainment Group.
Each of the six will be managed by its own CEO and board of directors.
Daniel Zhang will continue to serve as chairman and CEO of Alibaba Group, which will follow a holding company management model, the company said in a statement.
Zhang will also serve as CEO of Cloud Intelligence Group, as previously announced.
News of the restructuring comes one day after Alibaba founder Jack Ma was spotted at a primary school in Hangzhou, marking his first public appearance in mainland China in over a year.
Ma left China in late 2021 just as authorities launched a regulatory crackdown on the country's tech sector.