The two flights from Germany will be seen as a test for the Phuket Tourism Sandbox initiative, a trial scheme to reintroduce fully inoculated tourists to the island from July 1.
Businesses in tourism-reliant Phuket have been badly hit by the year-long Covid-19 travel ban.
The TG921 flights will depart Frankfurt at 2.45pm (local time) on April 2 (this Friday) and May 7 and arrive in Phuket at 7.10am the next day.