The Royal Irrigation Department has ordered them off Crown Property on the banks of Klong 13 so it can expand the canal and build a water gate.
Twenty representatives of the families submitted a written appeal to a complaint-receiving centre at Pathum Thani Provincial Hall on Tuesday morning.
Col Charas Wadkhien, head of the local office of Internal Security Operations Command, and Kanistha Sukcharoen, director of the complaint centre, accepted the letter and promised to get back to them within 20 days about assistance measures.
The squatters asked to be found new homes near their existing community so they could continue earning their living there.