The new hospital joins two Japanese hospitals, Takatsuki General and Sano, to offer patients high-quality healthcare services.
The Japanese Hospital by Samitivej falls under the management of Bangkok Dusit Medical Services (BDMS).
The hospital offers Japanese doctors and nurses and modern medical equipment.
Dr Chairat Panthuraamphorn, CEO of Samitivej and BNH Hospitals, said: "For many years, more Japanese expats have come to work in Southeast Asia, while Japanese investors have come to invest and establish companies in the EEC [Eastern Economic Corridor]."
Chairat said Samitivej Sukhumvit and Smitivej Sriraha Hospital have provided healthcare for the Japanese in the region for 40 years. Last year, there were about 400 Japanese patients per day and approximately 200 Japanese babies are born at Samitivej each year, Chairat added.